Figure 5. Effect of pregnenolone and mono-OH MXC co-treatment on mouse antral follicle atresia.
Isolated mouse antral follicles were cultured for 96 h with DMSO as a vehicle control, mono-OH MXC (10 μg/mL), pregnenolone (1 μg/mL), or mono-OH MXC and pregnenolone together. Following culture, follicles were processed for histological evaluation of follicular atresia as described in Materials and Methods. Data represent means ± SE from 3 separate experiments. Asterisks (*) indicate statistically significant differences (p<0.05) between treatments and vehicle.