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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Am J Transplant. 2013 Aug 6;13(10):2696–2702. doi: 10.1111/ajt.12404

Fig. 2. Allosensitization by donor splenocytes elicits lower DSA titers in eosinophil deficient BALB/c, but not B6 mice.

Fig. 2

A–B. Sera obtained from WT (black squares) and ΔdblGATA1 (white squares) BALB/c and B6 mice immunized with allogeneic B6 or BALB/c splenocytes (2 × 106 at d0 and d14), respectively, were tested by flow cytometry for binding to B6 thymocytes. A, % binding of each sera at various dilutions. (B) DSA titer (serum dilution at which binding to donor thymocytes is >5%) at 4w (top) and 12w (bottom) in WT and ΔdblGATA-1 BALB/c mice immunized with B6 splenocytes. (C) DSA titers at 4w (left) and 12w (right) from WT and ΔdblGATA-1 B6 mice immunized with BALB/c spleen cells. Similar trends in DSA titer were found at 20 weeks after B6 splenocyte injection or heart transplantation (not shown). Data are means ± SD. Binding to syngeneic BALB/c thymocytes was always <5% (not shown).