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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Am J Transplant. 2013 Aug 6;13(10):2696–2702. doi: 10.1111/ajt.12404

Fig. 5. Siglec-F antibody does not effectively deplete eosinophils.

Fig. 5

Representative flow plots of CD11b+ eosinophils in the bone marrow of BALB/c mice at 4 days after last treatment with vehicle control or Siglec-F antibody (clones E50-2440 and 238047; three 20μg i.p. injections every other day). Cells were gated on F4/80+Gr-1lo cells and (top) Siglec-F+ CD11b+ or (bottom) SSC and CD11b+. We obtained similar results administering 5 i.p. Siglec-F antibody injections every day, both in BALB/c and in B6 mice and looking either in the bone marrow or in the spleen (not shown).