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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Gastroenterology. 2013 Jul 25;145(5):966–969.e7. doi: 10.1053/j.gastro.2013.07.026

Figure 2. Cellular and viral determinants of MnHep cell HCV entry capacity.

Figure 2

MnHep cells transduced to express GFP alone as a negative control or the indicated HCV cell entry factors were challenged with GLuc expressing (A) HCVpp or (B) HCVcc. Infections were performed in parallel with an HCV neutralizing antibody (Anti-E2). GLuc values were measured 48 h.p.i. for HCVpp or the indicated times for HCVcc, normalized to parallel VSVGpp infections, and set relative to infection of highly permissive Huh-7.5 cells. Three independent experiments, each performed with six replicates, and statistics are relative to GFP alone. Naïve Huh-7.5 and MnHep cells were challenged with mouse CD81 adapted HCVcc and (C) intracellular and (D) extracellular HCV RNA levels were assayed as described above. Three independent experiments, each performed in triplicate. *** P<0.001 (Mann-Whitney test)