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Primers for DNA methylation analysis
| Genes | Modified Primer (5′→3′) | No. of CpG sites |
|---|---|---|
| Insr | F: GGGTTTTGGGGATAGTTTTGG R: CAATCCCTTCCCTACCTTAATCC |
32 |
| F: GGGAGGGTATTTGGGTTGGT R: CCTCTAAAATTAAAAACTCCACAAAAA |
24 | |
| Irs1 | F: TTGGAGTTAGGGGTAGGGATG R: CCCTCCTCCTCCTTCTCCTC |
21 |
| Glut4 | F: TGGTTAATGGGTGTTGTGAAGG R: AAACCCAACCCCCAAAATCT |
15 |
