Fig 1.

Single cell clone selection of Atp6v1c1-depleted 4T1 mammary tumor cells. A. RT-PCR assays of three mouse tissues and 4T1 cells were performed with gene-specific primers for Atp6v1c1, Atp6v1c2, and β-actin. B. Select GFP⁺ single subclones of 4T1 cells. The 4T1-LacZ clone expresses siRNA that specifically targets LacZ. The 4T1-c1s3-1, 4T1-c1s3-2 clones express siRNA that specifically targets C1. Visualized by white light (L) or by fluorescent light (FL) (primary magnification ×100). C. Anti-Atp6v1c1 immunostaining of different 4T1 cells as indicated (primary magnification ×100). D. Atp6v1c1, Atp6v1a and Atp6L expression in different 4T1 cells as indicated by Western blotting. E. Quantification of Atp6v1c1 protein expression level in different 4T1 cells as indicated (normalized to the tubulin level) (n=3). * P<0.05 compared with that of 4T1-LacZ cells. The cells shown were representative of the data (n=3).