Fig 3.
Atp6v1c1 knockdown inhibits 4T1 mouse mammary tumor growth and metastasis in vivo. A. Representative tumors from female BALB/c mice 28 days after subcutaneous inoculation in the left thoracic (#2) mammary gland fat pad with PBS or 1×105 4T1 cells infected (or not) as indicated (n=24). B. The mean tumor diameter (TD) of tumors during the period between post-implantation day 0 and day 26. Results are mean ± s.e.m. (n=24). * P<0.05 for 4T1-c1s3-1 compared with 4T1-LacZ by student's t-test at the endpoint. C. The weight of tumors on post-implantation day 28 (n=24). D. Bioluminescent Imaging (BLI) of mice as indicated (Red arrow indicates the metastatic site in BLI of mice and blue arrow indicates tumor necrosis). The images were representative of the data (n=12). Counts are photons detected. Images were captured with a 5min exposure. E. Whole body flux (photons/sec) quantification of mice injected with different 1×105 4T1 cells at day 16 as indicated (n=12). Signal measured from a 5 minute exposure. * P<0.05 for 4T1-c1s3-1 compared with 4T1-LacZ by student's t-test at the endpoint.