Figure 3.

FOXO1 suppresses basal transcription and GnRH induction of human FSHB-luc. (A–C) The −1028/+7 human FSHB-luc reporter was transfected into LβT2 cells along with pcDNA3 empty vector (EV) or FOXO1, as indicated. After overnight incubation in serum-free media, cells were treated for 6 hours with 0.1% BSA vehicle (Veh) or 10 nM GnRH. The results represent the mean ± SEM of three experiments performed in triplicate and are presented as luc/β-gal (A), basal transcription relative to empty vector (B), or fold GnRH induction relative to the vehicle control (C). *, FSHB-luc transcription is significantly repressed by FOXO1 compared to EV using Student's t test.