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. 2013 Sep 23;9(9):966–979. doi: 10.7150/ijbs.6996

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Fig 1 — The changes of endothelial permeability induced by IL-8 exhibited dose- and time-dependent manners. Permeability assay was perform as depicted in 2.1, and coefficient (P_a) in groups was calculated by formula P_a = [C_a]/t ×1/S ×V/[C_l]. (A) The transwell cultured with endothelium were treated with 50, 100 and 200 ng/ml IL-8, respectively. (B) The transwell cultured with endothelium were treated with 200 ng/ml IL-8 for 2, 4, 6 and 8h, respectively. Values represented the mean±S.E (n=3). *, P<0.05 denoted statistically significant difference compares to control.