Fig 8.

The effect of IL-8 on the protein expression of focal adhesion components, Paxillin, Vinculin and Talin. The expression levels of Paxillin, Vinculin and Talin were not changed by either concentration or duration of IL-8. (A) Representative Western blot bands of integrins expression. Quantification of each protein expression level by image analysis of the Western blot bands in fig A. The expression level of β-actin in each group was used as intrinsic controls, and relative expression were calculated. (B-D) The expression level of focal adhesion components, Paxillin, Vinculin and Talin with different doses of IL-8 (50, 100 and 200 ng/ml) for 2h. (E-G) The expression level of focal adhesion components with 100 ng/ml IL-8 for 2, 4, 6 and 8h, respectively. (H & I) The ratios of phosphorylated Paxillin (pPax) in total Paxillin (Pax) with different doses and treated durations of IL-8 were calculated and compared. Values represented the mean±S.D. (n=3).