Table 1.

Oligonucleotide primers used for RT-PCR analysis

Target transcript	Primer sequence (5'-3')	Product size (bp)
Occludin (forward)	5'-TTGGGACAGAGGCTATGG-3'	623
Occludin (reverse)	5'-ACCCACTCTTCA ACATTGGG-3'
Claudin-5(forward)	5'- CCTTCCTGGACCACAACATC -3'	203
Claudin-5 (reverse)	5'- GCCGGTCAAGGTAACAAAGA -3'
ZO-1 (forward)	5'- GCTAAGAGCACAGCAATGGA -3'	254
ZO-1(reverse)	5'- GCATGTTCAACGTTATCCAT -3'
β-actin (forward)	5'- TGACGTGGACATCCGCAAAG -3'	205
β-actin (reverse)	5'- CTGGAAGGTGGACAGCGAGG -3'