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. Author manuscript; available in PMC: 2013 Oct 23.
Published in final edited form as: Methods Cell Biol. 2013;115:327–342. doi: 10.1016/B978-0-12-407757-7.00020-7

Figure 3. Pictorial guide to CLEM processing for sectioning longitudinally to the spindle axis.

Figure 3

Following polymerization, resin was separated from the CLEM dish. Unwanted plastic was removed from the edges of the dish using pliers (A-C) allowing a razor to be inserted between the resin and the dish base (D). Following the separation of resin and dish (E) excess resin was removed using pliers (F) until just the capsule remained (G). The cell of interest was marked (H) with the aid of LM images (M) and resin coordinates (N). Unwanted resin was removed using a junior hacksaw (I) and a razor (J). Resin was trimmed using a microtome and a glass knife (K) until a neat block was generated at the top of a pyramid (L). Blocks were sectioned using a diamond knife (O) and ribbons collected using 100 mesh copper grids (P & Q), coated with formvar.

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