FIGURE 7.
Reduced intrinsic cell death propensity of mature activated and tissue-like memory B cell subsets from HCV-infected subjects. CD3-depleted PBMCs were prepared from peripheral blood samples and were stained with B cell subset markers (CD10, CD19, CD20, CD21, CD27, and CD38). B cell subsets were sorted by an FACSAria flow cytometer (BD Biosciences) and analyzed for baseline Annexin V staining as well as Annexin V staining after 20-h culture at 37°C. A, Immature transitional. B, Naive. C, Resting memory. D, Mature activated. E, Tissue-like memory. F, Plasma cells.