Fig. (3).

Niacin receptor characterization in macrophages. Panel A: Saturation Binding Assays: Increasing concentrations of [3H]- Nicotinic acid were incubated with 6 x 106 macrophages during 3 h at 25°C. Total (black) and non-specific (white, in the presence of 0.1 mM of Nicotinic acid) binding curves for [3H]-Nicotinic acid to isolated peritoneal macrophages from C57Bl/6J control mice were obtained. Panel B: Scatchard analysis of the results shown in panel A. The ratio of specific bound [3H]-Nicotinic acid to free (B/F) is plotted as a function of [3H]-Nicotinic acid bound (B). The intercept with the abscissa is the number of binding sites (Bmax) and the negative reciprocal of the slope is the dissociation constant (Kd). A representative experiment from 5 independent assays performed in duplicate is depicted in both panels. The Kd and Bmax values, mean ± S.E.M. from 5 experiments, for control and AGD mice macrophages are shown in the adjoined table. * p<0.05.