Table 1.

Effect of Pertussis Toxin (PTX) on Niacin (NA)-mediated Decrease of Stimulated Cytokine Secretion

Cytokine	Mice Condition	Treatment	Cytokine Levels (pg/ml)
IL-1β	Normal         AGD	LPS LPS+NA LPS+NA+PTX   LPS LPS+NA LPS+NA+PTX	3194 ± 303  2322 ± 267** 2995 ± 298#   3854 ± 321 2256 ± 287** 3764 ± 333#
TNF-α	Normal       AGD	LPS LPS+NA LPS+NA+PTX   LPS LPS+NA LPS+NA+PTX	3546 ± 294 2123 ± 189** 3721 ± 311#   4247 ± 395 2233 ± 227** 4323 ± 344#
IL-6	Normal       AGD	LPS LPS+NA LPS+NA+PTX   LPS LPS+NA LPS+NA+PTX	15442 ± 1670 10456 ± 998** 16028 ± 1687#   21234 ± 1711 10786 ± 1213** 19999 ± 1532#

Macrophages from control or AGD mice were pretreated with vehicle or PTX (50 ng/ml) for 18 h. After this time cells were incubated in the presence or absence of 1000 mM of Niacin (NA) and stimulated with LPS (10mg/ml) + IFN-g (100 U/ml) for 24 h. Cytokine levels were determined as described in Fig (4). Data are mean ± S.E.M. of three experiments in each group. **p<0.01 vs the corresponding basal value, # p<0.05 respect NA value.