Table 2.

Combination index values of high-density H929 cells pre-treated with SINE molecules for 24 hours followed by 24-hour incubation with additional myeloma chemotherapeutic drugs.

SINE	Chemo drug	CI (mean)	CI (SEM)	n
KPT185	Bortezomib	0.410	0.019	3
	Carfilzomib	0.322	0.136	3
	Dexamethasone	>2.0	*	5
	Doxorubicin	0.377	0.060	4
	Lenalidomide	>2.0	*	4
	Melphalan	0.849	0.104	4
KPT249	Bortezomib	0.888	0.166	3
	Carfilzomib	0.310	0.013	2
	Dexamethasone	1.263	0.193	4
	Doxorubicin	0.505	0.055	9
	Lenalidomide	1.098	0.186	3
	Melphalan	0.979	0.164	3
KPT330	Bortezomib	0.502	0.040	2
	Carfilzomib	0.482	0.043	2
	Dexamethasone	>2.0	*	2
	Doxorubicin	0.092	0.025	3
	Lenalidomide	>2.0	*	1
	Melphalan	0.687	0.254	3

Drug concentration ranges were as follows: SINE compounds KPT185, 249, and 330 were used at 4 nM to100 µM, doxorubicin at 4 nM to 100 µM, bortezomib at 40 pM to 10 µM, carfilzomib at 2 to 200 nM, and dexamethasone, melphalan and lenalidomide at 40 nM to 200 µM. *CI values and SEMs could not be calculated for several experiments due to the insufficient effects of single-agent lenalidomide or dexamethasone at the concentrations used. CI, combination index. The combination index method is based on that described by Chou and Talalay ²⁹. CI < 1, = 1, and >1 indicate synergism, additive effect, and antagonism, respectively.