Fig 5.

ASA blocks EGFR-activated cell viability by blocking phosphorylation of Erk and Akt in COX-1 expressing cells. Effects of aspirin on EGFR-activated cell viability in SKpcDNA (A) and SKCOX-1 cells (B). Cells were treated for 48 hours with aspirin (1 mmol/L) in the absence or presence of EGF (10 ng/ml). The cell viability assay was performed by using MTT, and values were normalized to untreated controls. Experiments were performed in triplicate and all data are shown as means ± SE. *, # Indicate a significant increase or decrease (p≤0.05), respectively, by Student's-t test. The inhibitory effect of aspirin on EGFR, Erk and Akt activation in SKpcDNA (C) and SKCOX-1 cells (D) by western blot analysis. Cells were pretreated with aspirin (1 mmol/L) for 24 hours followed by the addition of EGF (10 ng/ml) for 0-120 minutes. pEGFR, pErk and pAkt indicate phosphorylated EGFR, Erk and Akt, respectively. β-Actin was used as a loading control.