Table 1. Characteristics of the six microsatellite loci used in the study.
| Locus | Primers | Dye | [Primers] (μM) | Tm (°C) | Allele size range (bp) | Number of alleles | H′T | F′ST |
|---|---|---|---|---|---|---|---|---|
| A110 | F: 5′-CTATGGTGAATTTGTGATACAC-3′ | PET | 0.35 | 52 | 128–152 | 22 | 4.40 | 0.092 |
| R: 5′-ACCTTGTTGCATCTTTACC-3′ | ||||||||
| A125 | F: 5′-GCATATACATACCCGAGGTAAG-3′ | NED | 0.26 | 58 | 152–232 | 53 | 6.54 | 0.096 |
| R: 5′-AACCTGATGAAATGCACTATTC-3′ | ||||||||
| B4 | F: 5′-GGGCTCTGGCTCTGATAC-3′ | 6-FAM | 0.20 | 53 | 101–137 | 13 | 1.35 | 0.136 |
| R: 5′-TTGGATTAACCAACTTTCCTC-3′ | ||||||||
| B104 | F: 5′-GAACCTTATTCACTGGAATCTG-3′ | VIC | 0.30 | 53 | 122–188 | 30 | 4.13 | 0.122 |
| R: 5′-CCCTTTTCTTTCCTTTCTTAAC-3′ | ||||||||
| B123 | F: 5′-AATTTGCCTGACATTGTTACTC-3′ | NED | 0.22 | 53 | 206–269 | 48 | 6.35 | 0.116 |
| R: 5′-AGACCGTGTTCATTATGGTTAG-3′ | ||||||||
| C12 | F: 5′-GGAAAATGGGAAGTTCTAAGG-3′ | VIC | 0.30 | 50 | 120–320 | 16 | 1.77 | 0.155 |
| R: 5′-CCACAGAATTGAGGCAGTC-3′ |
H′T: phenotype-based diversity statistic of Obbard et al. (2006) calculated across all individuals.
F′ST: phenotype-based differentiation statistic of Obbard et al. (2006) between all 18 populations.