Figure 2.

Each of the 12 neuron classes in the fly lamina was targeted using the Split-GAL4 technique. Shown for each cell type are: single cell images illustrating defining features of each neuron class (A–L), a confocal section through an optic lobe of one Split-GAL4 driver (A′–L′), and a maximum intensity projection of expression in the central brain of the same Split-GAL4 line (A″–L″). Expression patterns were visualized by confocal microscopy using UAS driven expression of a membrane-targeted GFP and anti-GFP antibody staining (detailed in Supplemental Experimental Procedures). Blue labeling in single cell images shows a presynaptic marker (Brp; Nc82 antibody staining). Layer positions of terminals in the medulla are indicated as M1, M2 etc. in (A–L). For example, L1 has medulla terminals in layers M1 and M5 and L2 in layer M2. Each of the 12 lamina-associated neurons can be unambiguously identified by these anatomical features. Specific drivers used for each image are listed in Table S1. Ventral nerve cord expression patterns and images of the remaining Split-GAL4 lines are available on the authors’ website. Scale bars represent 50 μm (A′, A″, L) or 5 μm (all others).