Figure 6.
Regulation of the Hh, PDGFRα, IGF, and MAPK pathways in Hh-related tumors. Immunohistochemistry using an anti-PDGFRα antibody identifies strong PDGFRα staining in rhabdomyosarcomas (A) and medulloblastomas (B), relative to control skeletal muscle and cerebellar tissues. C, semiquantitative RT-PCR analysis of Hh-regulated mRNA expression in cultured primary medulloblastoma cells from CAGGS-CreER; R26-SmoM2 mice in the presence or absence of the SmoM2 inhibitor, KAAD-cyclopamine. Treatments were done for 72 hours. Descending wedge, serial dilutions of cDNA templates.