Abstract
A simple and rapid method for recovering enteroviruses from oysters is described. A polycation sewage flocculant promoted cohesion of oyster solids and thereby aided separation of these from the viruses. Recovery of 80 to 100% of experimentally inoculated virus was achieved, and the suspension or extract obtained could be inoculated directly into tissue cultures or concentrated first for greater sensitivity.
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Selected References
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- Cliver D. O., Herrmann R. M. Economical tissue culture technics. Health Lab Sci. 1969 Jan;6(1):5–17. [PubMed] [Google Scholar]
- Grindrod J., Cliver D. O. A polymer two phase system adapted to virus detection. Arch Gesamte Virusforsch. 1970;31(3):365–372. doi: 10.1007/BF01253770. [DOI] [PubMed] [Google Scholar]
- Grindrod J., Cliver D. O. Limitations of the polymer two phase system for detection of viruses. Arch Gesamte Virusforsch. 1969;28(3):337–347. doi: 10.1007/BF01240948. [DOI] [PubMed] [Google Scholar]
- HEDSTROEM C. E., LYCKE E. AN EXPERIMENTAL STUDY ON OYSTERS AS VIRUS CARRIERS. Am J Hyg. 1964 Mar;79:134–142. doi: 10.1093/oxfordjournals.aje.a120369. [DOI] [PubMed] [Google Scholar]
- Hamblet F. E., Hill W. F., Jr, Akin E. W., Benton W. H. Oysters and human viruses: effect of seawater turbidity on poliovirus uptake and elimination. Am J Epidemiol. 1969 May;89(5):562–571. doi: 10.1093/oxfordjournals.aje.a120969. [DOI] [PubMed] [Google Scholar]
- Herrmann J. E., Cliver D. O. Methods for detecting food-borne enteroviruses. Appl Microbiol. 1968 Oct;16(10):1564–1569. doi: 10.1128/am.16.10.1564-1569.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MASON J. O., McLEAN W. R. Infectious hepatitis traced to the consumption of raw oysters. An epidemiologic study. Am J Hyg. 1962 Jan;75:90–111. doi: 10.1093/oxfordjournals.aje.a120238. [DOI] [PubMed] [Google Scholar]
- METCALF T. G., STILES W. C. THE ACCUMULATION OF ENTERIC VIRUSES BY THE OYSTER, CRASSOSTREA VIRGINICA. J Infect Dis. 1965 Feb;115:68–76. doi: 10.1093/infdis/115.1.68. [DOI] [PubMed] [Google Scholar]
- Metcalf T. G., Stiles W. C. Enteroviruses within an estuarine environment. Am J Epidemiol. 1968 Nov;88(3):379–391. doi: 10.1093/oxfordjournals.aje.a120898. [DOI] [PubMed] [Google Scholar]
- Mitchell J. R., Presnell M. W., Akin E. W., Cummins J. M., Liu O. C. Accumulation and elimination of poliovirus by the eastern oyster. Am J Epidemiol. 1966 Jul;84(1):40–50. doi: 10.1093/oxfordjournals.aje.a120626. [DOI] [PubMed] [Google Scholar]
- ROOS B. Hepatitepidemi, spridd genom ostron. Sven Lakartidn. 1956 Apr 20;53(16):989–1003. [PubMed] [Google Scholar]
