Figure 4. Epi-SRS images of fresh brain slices from normal mouse and human GBM xenograft (BT112) mouse model.

Images are representative of 10 normal mice (A to H) and 6 BT112 mice (I to M). Lipids have been assigned to the green channel and proteins to the blue channel. Fresh 2-mm thick coronal section of normal mouse brain. Structural features of the full section from a normal mouse (A), cortex (B), hippocampus (C), corpus callosum (D), choroid plexus (E), hypothalamic nuclei (F), habenular nucleus (G), and caudatoputamen (H) demonstrate the expected histoloarchitectural patterns. In contrast, fresh 2-mm-thick coronal brain section of a BT112 human glioblastoma xenograft-bearing mouse reveals normal, green appearing brain parenchyma surrounding blue hypercellular tumor (I). High magnification of the tumor core reveals individual tumor cells (J). The tumor-gray matter interface (white dashed line) demonstrates an invasive pattern of tumor growth (K). The tumor-white matter interface, demonstrates the ability of tumor cells (blue) to traverse and separate white matter bundles (green) (L). A line profile of S₂₉₃₀/S₂₈₄₅ across the gray matter/tumor interface in (K, red dot-dashed line) shows higher S₂₉₃₀/S₂₈₄₅ with increasing tumor density (M).