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. Author manuscript; available in PMC: 2013 Oct 23.
Published in final edited form as: J Phys Chem C Nanomater Interfaces. 2012 Sep 17;116(40):21563–21571. doi: 10.1021/jp3072876

Table 1. Spectral Properties of Streptavidin Conjugated Dyes in Solution and When Bound to the Multilayered Substrate.

SA-dye conjugate AF488 AF647 AF680 AF750 IRD800
λexc/λems (nm) 495/519 650/665 679/702 749/775 795/819
ε (M−1 cm−1) 71 000 239 000 184 000 240 000 207 000
QY in solution 0.4233 0.3333 0.30 0.12 0.1532
〈τ〉a in solution (ns) 2.71 1.65 1.54 0.87 0.81
〈τ〉 on glass (ns) 1.06 0.77 0.85 N/A N/A
〈τ〉 on substrate (ns) 0.28 0.023–0.078 0.017–0.071 0.033–0.072 0.015–0.055
a

Amplitude weighted lifetime (〈τ〉 = Σαiτi where αi is the pre-exponential factor and Σαi = 1).