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. Author manuscript; available in PMC: 2013 Oct 23.

Published in final edited form as: Gastroenterology. 2009 Dec 21;138(3):1166–1177. doi: 10.1053/j.gastro.2009.12.005

Shh misdirects the pancreatic ductal epithelium toward a GI mucinous metaplasia. (A) Shh exposure results in enhanced mucin expression (PAS: magenta, arrowheads) in HPDE cells. Real-time quantitative RT-PCR reveals that HPDE cells are Hh target cells expressing the receptor PTCH2. In response to Shh exposure the pathway is activated (PTCH2 upregulation) along with upregulation of gastric mucins (MUC5ac, MUC6). (B) Transgenic misexpression of Shh in Pdx-Shh mice results in a GI phenotype of pancreatic ducts with expression of PAS⁺ (magenta) and Alcian blue⁺ mucins, including Muc5ac. Scale bars indicate 100μm.

Shh misdirects the pancreatic ductal epithelium toward a GI mucinous metaplasia. (A) Shh exposure results in enhanced mucin expression (PAS: magenta, arrowheads) in HPDE cells. Real-time quantitative RT-PCR reveals that HPDE cells are Hh target cells expressing the receptor PTCH2. In response to Shh exposure the pathway is activated (PTCH2 upregulation) along with upregulation of gastric mucins (MUC5ac, MUC6). (B) Transgenic misexpression of Shh in Pdx-Shh mice results in a GI phenotype of pancreatic ducts with expression of PAS⁺ (magenta) and Alcian blue⁺ mucins, including Muc5ac. Scale bars indicate 100μm.