Figure 3.

Effect of TKE and CuD on the expression of apoptosis-related molecules. ((a), (b)) MDA-MB-231 cells were treated with 60 μg/mL TKE or 1 μg/mL CuD for the indicated time periods, and the cell lysates were subjected to western blot analysis using specific antibodies. ((c), (d)) MDA-MB-231 cells were incubated in the absence or presence of TKE or CuD and submitted to TUNEL assay. For the TUNEL assay, cells were fixed, permeabilized, and visualized for DNA degradation using dUTP-labeling. Red fluorescence: nuclei stained with PI. Green or yellow (resulting from merged red and green) fluorescence: apoptotic nuclei containing fragmented DNA.