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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Cytokine. 2013 Aug 26;64(2):10.1016/j.cyto.2013.08.001. doi: 10.1016/j.cyto.2013.08.001

Fig. 2. IL-6 signaling induces expression pro-survival and pro-metabolic factors.

Fig. 2

A) Pro-survival factor expression in IL-6 stimulated T cells. Purified LN T cells were incubated overnight in medium, IL-7 or IL-6. mRNA expression of the indicated genes were assessed by qRT-PCR. Data show the mean +/− SEM of three independent experiments.

(B) Immunoblot analysis of Mcl-1 expression in cytokine stimulated cells. Purified LN T cells were incubated with IL-6, IL-7 or in medium alone for 24 hours, and total cell lysates were probed for Mcl-1 and then reprobed for β-actin as loading control. Numbers in box indicate relative Mcl-1 expression as determined by densitometry of the bands. Blot is representative of two independent experiments.