Fig. 5. T cell quiescence is maintained in IgH^KOIL6^Tg mice.

(A) Surface CD69 and CD25 expression on IgH^KO and IgH^KOIL6^Tg T cells. Histograms are representative of three independent analyses with each one mouse.

(B) Activated/memory phenotype cells percentages in CD4 and CD8 LN T cells. Contour plots of surface CD62L and CD122 versus CD44 expression are representative of three independent analyses with each one mouse.

(C) Ki-67 intra-nuclear staining of CD4 LN T cells from IgH^KO and IgH^KOIL6^Tg mice. Histogram is representative of and bar graph shows the mean +/− SEM from three independent experiments with each one mouse.

(D) BrdU incorporation in DN thmocytes and LN T cells from IgH^KO and IgH^KOIL6^Tg mice. After initial i.p. injection with BrdU, mice were supplied with BrdU in drinking water for 3 days. BrdU incorporation was assessed by anti-BrdU intracellular staining. Bar graph shows the mean +/− SEM for the indicated cells from two independent experiments with two IgH^KO and five IgH^KOIL6^Tg mice.

(E) FoxP3⁺ and Helios⁺ CD4 T cells in IgH^KO and IgH^KOIL6^Tg mice. Contour plots are representative of three independent analyses with each one mouse.

(F) FoxP3⁺CD25⁺ CD4 Treg cell percentages in IgH^KO and IgH^KOIL6^Tg LN cells. Data show the mean +/− SEM percentages of FoxP3⁺CD25⁺ cells among CD4 LN T cells from three independent experiments.