Table 1.
The top 40 differentially expressed genes in response to heat shock stress in Clostridium botulinum ATCC 3502.
| Locus tag | COG | Gene name | Function class | Fold change | Function description |
|---|---|---|---|---|---|
| Posttranslational modification, protein turnover, and chaperones [O] | |||||
| CBO2958 | COG0484 | dnaJ | [O] | +8.9 | Chaperone protein |
| CBO2959 | COG0443 | dnaK | [O] | +17.2 | Chaperone protein |
| CBO0189 | COG0484 | [O] | +8.2 | DnaJ-related protein | |
| CBO2960 | COG0576 | grpE | [O] | +5.6 | Heat shock protein |
| CBO3299 | COG0234 | groES | [O] | +14.2 | 10 kDa chaperonin GroES |
| CBO3298 | COG0459 | groEL | [O] | +8.3 | 60 kDa chaperonin GroEL |
| CBO0501 | COG2077 | tpx | [O] | +5.0 | Thiol peroxidase |
| CBO0803 | ha-33 | +1.8 | Toxin cluster gene ha-33 | ||
| CBO0802 | ha-17 | +1.6 | Toxin cluster gene ha-17 | ||
|
| |||||
| Transcription [K] | |||||
| CBO0645 | COG1846 | [K] | +16.1 | MarR-family transcriptional regulator | |
| CBO1012 | COG1846 | [K] | +7.7 | MarR-family transcriptional regulator | |
| CBO1017 | COG4978 | [KT] | −5.8 | MerR-family transcriptional regulator | |
| CBO2304 | COG1695 | [K] | +5.8 | Transcriptional regulator, PadR family | |
| CBO0786 | COG0745 | [KT] | +4.4 | Two component response regulator | |
| CBO0100 | COG1609 | ccpA | [K] | −7.3 | LacI-family transcriptional regulator |
| CBO2938 | COG0568 | rpoD | [K] | +4.2 | RNA polymerase sigma factor (sigma-43) |
| CBO3519 | COG0782 | greA | [K] | −5.0 | Transcription elongation factor |
|
| |||||
| Translation and ribosomal structure and biogenesis [J] | |||||
| CBO3164 | COG0525 | valS | [J] | −12.1 | Valyl-tRNA synthetase |
| CBO3069 | COG0809 | queA | [J] | −9.2 | tRNA ribosyltransferase-isomerase |
| CBO1204 | COG0162 | tyrR | [J] | −5.6 | Tyrosyl-tRNA synthetase |
| CBO3265 | COG0064 | gatB | [J] | −4.3 | Glutamyl-tRNA amidotransferase subunit B |
| CBO3503 | COG0442 | proS2 | [J] | −4.3 | Prolyl-tRNA synthetase |
| CBO3323 | COG0162 | tyrS | [J] | −4.7 | Tyrosyl-tRNA synthetase |
| CBO3470 | COG0198 | rplX | [J] | −6.7 | 50S ribosomal protein L24 |
| CBO3474 | COG0092 | rpsC | [J] | −4.6 | 30S ribosomal protein S3 |
| CBO0400 | COG0465 | ftsH | [J] | −6.3 | Cell division protein |
|
| |||||
| Amino acid transport and metabolism [E] | |||||
| CBO1675 | COG0747 | [E] | +6.8 | Oligopeptide-binding protein | |
| CBO1860 | COG0697 | ecsC | [E] | +4.1 | Membrane protein |
|
| |||||
| Cell wall, membrane, and envelope biogenesis [M] | |||||
| CBO2101 | COG1087 | [M] | −4.2 | UDP-glucose epimerase | |
| CBO3597 | COG0764 | fabZ | [M] | +8.3 | (3R)-Hydroxymyristoyl-ACP dehydratase |
|
| |||||
| Hypothetical proteins | |||||
| CBO3522 | −4.1 | Hypothetical protein | |||
| CBO2185 | +5.3 | Hypothetical protein | |||
| CBO2640 | −6.3 | Hypothetical protein | |||
| CBO2096 | +5.8 | Hypothetical protein | |||
| CBO3353 | +7.6 | Hypothetical protein | |||
|
| |||||
| Function unknown [S] | |||||
| CBO1070 | COG3862 | [S] | +6.0 | Hypothetical protein | |
| CBO0014 | COG3862 | [S] | −4.2 | Hypothetical protein | |
| CBO2935 | COG0327 | [S] | +4.1 | Hypothetical protein | |
|
| |||||
| General function prediction only [R] | |||||
| CBO1449 | COG2234 | [R] | +4.6 | Membrane protein | |
| CBO0670 | COG2984 | [R] | −4.2 | Lipoprotein | |
| CBO2137 | COG2234 | [R] | +4.7 | Membrane protein | |
|
| |||||
| Cell motility [N] | |||||
| CBO2645 | COG1298 | flhA | [NU] | −7.9 | Flagellar biosynthesis protein |
| CBO2652 | COG1291 | motA | [N] | −5.2 | Chemotaxis MotA protein |
|
| |||||
| Energy production and conversion [C] | |||||
| CBO3577 | COG0778 | [C] | −5.2 | Nitroreductase | |
|
| |||||
| Signal transduction mechanisms [T] | |||||
| CBO0787 | COG0642 | [T] | +6.5 | Two-component sensor kinase | |
| CBO1120 | COG0642 | [T] | +4.3 | Two-component sensor kinase | |
|
| |||||
| Cell cycle control, cell division, and chromosome partitioning [D] | |||||
| CBO2535 | COG0206 | ftsZ | [D] | −4.7 | Cell division protein FtsZ |
|
| |||||
| Coenzyme transport and metabolism [H] | |||||
| CBO2911 | COG0422 | thiC | [H] | −4.3 | Thiamine biosynthesis protein |
| CBO2601 | COG1057 | [H] | +4.6 | Transferase | |
|
| |||||
| Nucleotide transport and metabolism [F] | |||||
| CBO0132 | COG0504 | pyrG | [F] | −4.7 | CTP synthase |
|
| |||||
| Carbohydrate transport and metabolism [G] | |||||
| CBO1071 | COG2271 | glpT | [G] | +4.3 | Glycerol-3-phosphate transporter |
|
| |||||
| Secondary metabolites biosynthesis, transport, and catabolism [Q] | |||||
| CBO1169 | COG2313 | [Q] | +4.3 | Indigoidine synthase A family protein | |