FIGURE 5.
Analysis of alloreactive CD4+ and CD8+ T cell proliferation in vivo. CFSE-labeled CD8−/− C57BL/6 (a) and CD4−/− C57BL/6 (b) lymphocytes were injected through the lateral vein into irradiated BALB/c recipient mice. Recipient mice were then untreated or treated with CTLA4/Fc (0.1 mg), IL-15 mutant/Fcγ2a (1.5 µg) or a combination of both fusion proteins for 3 days. a, CFSE-labeled allogeneic CD4+ T cells from the host spleen. b, CFSE-labeled allogeneic CD8+ T cells from the host spleen. Data are represented as percentage of proliferation of CFSE-labeled CD4+ and CD8+ T cells. Data are representative of four individual experiments.