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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Immunol. 2013 Sep 20;191(9):4514–4520. doi: 10.4049/jimmunol.1202603

FIGURE 3. Increased eosinophil recruitment in CRA-challenged abr−/− mice.

FIGURE 3

(A) Eosinophil counts in BAL. (B) Eosinophil peroxidase (EPO) levels in cell-free BAL fluid. A–B, data of one experiment were shown. (C) Total peribronchial eosinophils counts at 50 high-power fields (under ×400 magnification) of H&E stained sections. Results representative of two independent experiments. For A–C, data are expressed as mean ±SEM and analyzed by unpaired Student’s t test (*, p<0.05; **, p<0.01). n=3–4 mice per genotype. (D) Adoptive transfer of eosinophils. Eosinophils (SSChiCD11b+Ly6G−) flow-sorted from single cell suspensions of lungs on day 21 following the immunization/challenge protocol in Fig. 2A were transferred into naïve wt mice, followed by intratracheal CRA challenge at 24 h and 72 h after adoptive transfer. Airway hyper-responsiveness was measured 24 h after the final CRA challenge. Data of one experiment were analyzed by one-way ANOVA (*, p<0.05). n = 3 mice per group.