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. 2013 Sep 3;91(9):568–575. doi: 10.1038/icb.2013.43

Figure 1.

Figure 1

Activation of AhR changes maturation of precursor DCs. Activation of AhR increases maturation of precursor DCs derived from B6 wt (AhR+/+) but not in BMDCs from AhR-null (AhR−/−) mice. Bone marrow cells were cultured for 7 days in RPMI containing 20 ng ml−1 GM-CSF with 0.1% DMSO (vehicle control, a and c) or 1 nM TCDD (b and d). Generation of BMDCs was carried out as described earlier.27 Representative flow cytometric analysis dot plots of CD11c and class II MHC coexpression on propidium iodide-negative cells are shown. Dot plots are representative of three independent experiments. Cells within region R7 indicate DC subset of double-negative cells (DN), R6 precursor DC, R5 IDC and R4 indicate MDC. The mean of the percentage of each DC subset is shown within each region from three independent experiments. The total yield of nonadherent cells was 2.6±0.5 × 106 (AhR+/+ Control), 2.2±0.4 × 106 (AhR+/+ TCDD), 2.3±0.7 × 106 (AhR−/− Control) and 2.4±0.3 × 106 (AhR−/− TCDD). Data are presented as means±s.e.