Figure 6.
Pretreatment with α-ZAL reduced the expression of proapoptotic protein Bax in homocysteine-challenged HUVECs. The expression of proapoptotic protein Bax was significantly increased after treatment with 500 μmol/L homocysteine for 24 h. Pretreatment with α-ZAL could reduce the expression of Bax. (a) Immunohistochemistry staining (only showing the picture of the highest concentration 10−6 mol/L). (b) Western blot. Data were presented as mean ± SD. *P < 0.05 versus Vehicle, # P < 0.05 versus Hcy alone. α-ZAL: α-zearalanol; 17β-E2: 17β-estradiol; Hcy: homocysteine.