Figure 5.
Total β 2-AR expression in DLN cells from AA and non-AA rats at (a) D21 and (b) D28 after immune challenge ((a)–(c)). In contrast to splenocytes, expression of β 2-AR was unchanged in DLN cells from CFA-, SMB-, and MO-challenged rats on D21 or D28 compared with Saline-treated controls. DLN cells were harvested, lysed, and proteins resolved by SDS-PAGE. The data were normalized to β-actin. Cellular extracts were probed with a β 2-AR receptor antibody, and β 2-AR expression was quantified by densitometry. An example of the western blots at D28 is shown in (c). Each bar represents the mean optical density ± SEM with an n of 8 rats per treatment group. A one-way ANOVA with Bonferroni multiple comparison tests was used to determine statistically significant differences between treatment groups.