Table 1.

Properties of Commonly Used Fluorescent Proteins

FP	Monomeric	pKa	N-linked consensus seq.	Disulfide forming
EGFPa	No	6.0	No	Yes
mGFPa–c	Yes	6.0	No	Yes
mVenusd	Yes	6.0	No	Yese
mCitrinef	Yes	5.7	No	Predicted
Ceruleanc,g	Yes	3.2	No	Yese
TagBFPh,i	Yes	2.7	Yes	Yes
TagRFPa	No	<4.0	Yes	Predicted
mCherrya	Yes, but sticky	<4.5	No	No
sfGFPc,j	Yes	5.5	No	No

^aShaner et al. (2007).

^bSnapp et al. (2003).

^cAronson et al. (2011).

^dNagai et al. (2002).

^eOur unpublished results.

^fGriesbeck et al. (2001).

^gMarkwardt et al. (2011).

^hCostantini et al. (2013).

ⁱSubach et al. (2008).

^jPedelacq et al. (2006).

FP, fluorescent protein; sfGFP, superfolder green fluorescent protein.