FIG. 5.

Response to knockdown of agrp in the Alamar Blue assay. Three fish/well were incubated in 4 mM sodium bicarbonate with 1% Alamar Blue. Fluorescence of the solution was measured at time 0 (4 days postfertilization; dpf) and 24 h later (5 dpf). Data are reported as the relative change in fluorescence (mean±SEM). Numbers within the bar indicate number of wells (n). (A) Response to injection of a standard control morpholino used as the control (0) treatment in the agrp morpholino studies, (B) agrp morpholino dose response in wild-type fish. (C) agrp morpholino dose response in mc4r −/− fish. (D) Response to 1.6 ng/embryo of agrp morpholino in fish injected with 1.6 ng/embryo standard control morpholino (clear) or 1.6 ng/embryo mc3r morpholino (black). Numbers within each bar indicate the number of samples (n). ^a,bBars that do not share a superscript differ significantly (p<0.05).