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. 2013 Sep;10(3):343–352. doi: 10.1089/zeb.2012.0841

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Copyright 2013, Mary Ann Liebert, Inc.

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FIG. 6. — Relative luminescence units (RLU) of human embryonic kidney (HEK293) cells expressing human (h) or zebrafish (z) melanocortin 3 receptors (MC3R). (A) When RLU are corrected for responsiveness to Forskolin, HEK293 cells that express zMC3R display high levels of constitutive activity (IBMX treatment) that is blocked by agouti-related protein (AgRP) treatment. (B) AgRP dose dependently decreases RLU in IBMX-treated cells that express zMC3R. ^a,b,cColumns with different superscripts differ significantly from other columns that signify treatment of cells expressing the same receptor (p<0.05. n=4).