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Real time PCR analysis of the expression of proinflammatory cytokines in HUVECs. HUVECs were infected with AdGFP or AdRas12V or treated with 100 μmol/L H2O2. After 1 week, total RNA was extracted for real time PCR analysis. The expression of IL1-β, IL-6, and NGF was normalized to GAPDH. ∗ and ∗∗: P < 0.05 and P < 0.01, respectively, versus AdGFP infection. †: P < 0.01 versus AdRas12V infection (n = 6 per group).
