Figure 5.

hMSC differentiation assays. MSCGM (black) as well as MSCGM-CD (white)-cultured cells revealed to be 100% positive for expression of osteogenic and chondrogenic lineage markers. Commitment along adipogenic lineage occurred in 13.51% of MSCGM-CD and in 18.37% of MSCGM propagated cells. Cells were counted in 10 high power fields that contained at least 100 cells, and the amount of nuclei (representing total amount of cells) was compared with the amount of cells positive for the differentiation marker. Data presented as mean ± SEM (n = 5). Unpaired two-tailed t-test demonstrated no significant differences between both groups.