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. 2013 Oct 1;2013:706156. doi: 10.1155/2013/706156

Figure 3.

Figure 3

(a) ConA induced the release of cytokines in untreated (blank), negative-control-treated, and Nec-1-treated mice. IL2, IL4, IL6, IL10, TNF-α, IFN-γ, and GAPDH mRNA expression in the livers of untreated (blank), negative-control-treated, and Nec-1-treated mice. IL2, IL6, IL10, TNF-α, and IFN-γ mRNA expression in the livers of untreated (blank) and negative-control-treated groups were significantly increased after ConA injection compared with those in the Nec-1-treated mice. (b) Effects of Nec-1 on ConA-induced hepatic IFN-γ, IL2, and IL6 protein expression. (c) Localization of IL2 and IL6 in the liver tissues detected with immunofluorescence (original magnification ×200). These experiments were repeated three times, and representative results are shown.