Figure 6.
PsA inhibits HUVEC Cell Migration. Quantitative scratch assay of HUVEC migration from microscopic images taken at 400× magnification. Serum deprived HUVEC with 0.05% HPβCD in complete growth media (light grey); 0.05% HPβCD in depleted media (dark grey); 3.9 × 10−8 M PsA in 0.05% HPβCD (black) over 10 h at 37 °C. * Indicates significance of p value <0.01. Data are presented as mean ± SEM (n = 16). Note that it is impossible to perform these experiments using PsA alone i.e., without DMSO or Cremophor, or the cyclodextrin being present, due to its low solubility in an aqueous medium.