Table 1.
Effects of various pretreatments on TE-induced concentration-contraction response curves in isolated aortic rings.
| Pretreatments | Endothelium | EC50 (ug/mL) | Emax (% KCl) | n |
|---|---|---|---|---|
| No drug | + | 18.25 ± 0.13 | 81.33 ± 5.56 | 7 |
| l-NAME(1 μM) | + | 12.13 ± 0.12 * | 97.80 ± 9.81 * | 7 |
| Indomethacin (1 μM) | + | 18.42 ± 0.14 | 76.21 ± 5.64 | 7 |
| No drug | − | 11.93 ± 0.09 * | 92.91 ± 8.15 * | 7 |
| Low-calcium | − | 18.74 ± 0.47 # | 33.59± 3.62 # | 6 |
| Calcium-free | − | 25.89 ± 6.14 # | 4.97 ± 3.54 # | 6 |
| Diltiazem (1 μM) | − | 18.65 ± 0.36 # | 40.17 ± 7.27 # | 6 |
| Nifedipine (1 μM) | − | 18.52 ± 0.27 # | 55.67 ± 5.22 # | 6 |
| Verapamil (1 μM) | − | 18.72 ± 0.39 # | 49.25 ± 5.16 # | 6 |
| 2-APB (10 μM) | − | 12.59 ± 0.32 | 55.30 ± 11.92 # | 6 |
| Heparin (2500 IU) | − | 36.03 ± 0.18 # | 49.57 ± 5.63 # | 6 |
| PKC 412 (5 μM) | − | 12.15 ± 0.12 | 85.94 ± 13.93 | 6 |
| U-73122 (10 μM) | − | 12.11 ± 0.19 | 82.70 ± 10.47 | 6 |
| Propranolol (1 μM) | − | 12.15 ± 0.12 | 89.59 ± 10.71 | 6 |
| Phentolamine (1 μM) | − | 11.81 ± 0.07 | 94.49 ± 13.02 | 6 |
TE, tentacle extract; l-NAME, Nω-nitro-l-arginine methyl ester; 2-APB, 2-aminoethoxydiphenyl borate; EC50, the concentration (μg/mL) required to produce 50% maximum response; n, number of arteries (each taken from separate rats). * p < 0.05 vs. no drug with endothelium; # p < 0.05 vs. no drug without endothelium; one-way ANOVA with Dunnett’s posttest for multiple comparisons.