Table 2.

Cytotoxicity IC₅₀ values (µg mL⁻¹) for the crude extract, DCM and aq.MeOH fractions of V. rugosum crude extract (N = 2).

IC50 (µg mL−1): median (values obtained with two independent experiments)
	Neuro2A cells (24 h of incubation)	KB cells (72 h of incubation)	Undifferentiated Caco-2 cells (48 h of incubation)
Crude extract	0.38 (0.24–0.51)	0.19 (0.26–0.12)	N.R. (40%)
DCM fraction	0.15 (0.14–0.15)	0.007 (0.006–0.008)	N.R. (40%)
aq.MeOH fraction	0.03 (0.01–0.04)	0.004 (0.003–0.004)	N.R. (45%)

N.R.: no IC₅₀ was observed and maximum cytotoxicity at 5 µg mL⁻¹ indicated in brackets.