Table 3.
Cytotoxicity IC50 values (ng mL−1) for the 5 fractions obtained using SPE cartridge (N = 2).
| IC50 (ng mL−1): median (values obtained with two independent experiments) | |||||
|---|---|---|---|---|---|
| Fraction Weight (%) |
Distribution of PnTX-G (%) |
KB cells (72 h of incubation) |
Neuro2A cells (24 h of incubation) |
Undifferentiated Caco-2 cells (48 h of incubation) |
|
| F1 | 4.9 | 0 | 1023 (958, 1088) | N.R. (45%) | N.R. (44.5%) |
| F2 | 50.3 | 0 | 0.21 (0.18, 0.24) | 11 (5–17) | N.R. (41.6%) |
| F3 | 26.5 | 100 | 2.59 (2.55, 2.63) | 25 (44–6) | N.R. (42.6%) |
| F4 | 4.6 | 0 | 53.6 (45.4, 61.8) | 575 (760–390) | N.R. (25.2%) |
| F5 | 13.8 | 0 | 62.7 (55.9, 69.5) | 1140 (800–1480) | N.R. (41.1%) |
N.R.: no IC50 was observed and maximum cytotoxicity at 5 µg mL−1 indicated in brackets.