Transcript-specific RT qPCR was performed to measure levels of noncoding transcripts associated with coding genes. Signals were normalized to RDN25-1 rRNA levels, and expressed as fold difference from WT ncRNA signal. Error bars represent 1 SD from technical replicates.
(A) Relative levels of SUT2593 and RPS6A transcript are shown.
(B) Relative levels of SUT258 and RPL10 transcript are shown.
(C) Relative levels of ncRNA were measured in cultures at 10 hr of sporulation for WT and jhd2Δ.