Fig. 2.

Expression of class III NnSR1 transcript induced by Pi deprivation in Nicotiana alata roots. (A) RT-PCR amplification from roots exposed to 14 d of Pi deprivation. Degenerate primers designed from C2 and C5 conserved domains of Nicotiana S-RNases were used. (B) Similar to (A) but using NnSR1-specific primers. (C) RT-PCR amplification using NnSR1-specific primers from roots. Numbers indicate the days of exposure to Pi starvation. Representative gels and the statistical analysis are shown. Signal intensity values represent the mean ± s.e.m. of three independent experiments. Data were analysed using the t-test (A, B) or one-way ANOVA followed by Tukey test (C). ***P < 0·001. Pi-, Pi-deficient medium; Pi+, Pi-sufficient medium.