Table 1.

Changes in gene expression estimated by microarray hybridization and by quantitative real-time RT–PCR

		Fold change average in microarray*		Fold change average in qRT–PCR†
Unigene ID	Primers	Rough lemon	Pineapple seet orange	Duncan grapefruit	Rough lemon	Murcott tangor	Pineapple sweet orange
CTF0607	5′-AAGCCATGGCAGCAATCG-3′	4·70	3·98	6·41	7·40	6·94	6·29
	5′-GATGTCTGAACTCTGGCCACTT-3′
CTF0899	5′-AACTGCTATGACATGGAAAACATCA-3′	2·17	1·85	1·58	1·62	1·68	1·30
	5′-CCTGAACCCAGTTGTTGAAGTCT-3′
CTF0931	5′-GGACATCTGAATGAAATTGATGCT-3′	7·00	1·48	2·90	2·80	6·12	1·93
	5′-CATGCTGTTTCAATCTCAGGTTCT-3′
CTF0516	5′-AAGTTCCTCCTCATCGACTAGCA-3′	2·47	3·14	1·93	1·89	2·70	2·03
	5′-TGATTGAACTGGGTAGCGATTG-3′
CTF0558	5′-CGAATGAGGTCTCGAGAAGCA-3′	1·57	2·05	5·39	4·83	6·67	1·94
	5′-AATCTTGATCCCTTCAAAATCGAA-3′
CTF0173	5′-AAAGAAGGTAAAAGAAAAGGAGAAGCT-3′	6·33	13·05	10·02	7·00	3·79	14·25
	5′-GGATGAGTTCCAGTCATGATTTAGC-3′
CTF0316	5′-CGCTACCAGAGTCGCATTACAG-3′	2·87	3·17	7·22	5·27	3·10	1·63
	5′-AGTTGCAGGCTCGAATATCCA-3′
CTF0397	5′-CGATCCCTGCGGTATCAAGT-3′	2·37	1·41	3·90	2·55	3·58	3·32
	5′-GCTCATCTGAACCAGAGTTTGAGTT-3′

*Average fold change of the mean of expression values of four replicates in the microarray in adult samples with respect to juvenile samples.

^†Average fold change of three independent RT–PCRs for three independent samples for each variety.