FIG. 2.

β-Klotho is downregulated in islets of adult db/db mice. mRNA levels of FGFRs and β-klotho (A) were determined in 12-week-old m+/db and db/db mice. mRNA (B) and protein (C) levels of β-klotho in islets were analyzed in 4-, 8-, and 12-week-old mice. For the Western blot, β-actin was used as a loading control. Densitometry is shown and was calculated as β-klotho/β-actin. Data are means ± SEs. *P < 0.05; **P < 0.01; ***P < 0.001 vs. age-matched m+/db group; #P < 0.05; ##P < 0.01 vs. 4-week db/db group (n = 4 to 5).