FIG. 3.

CD11b⁺/CD11c^– cells are the main population expressing the suppressive enzymes and Ym1/Ym2. Female Proins2^−/− mice at 5–6 weeks of age were inoculated intraperitoneally with a single dose of either CVB4 or PBS and treated with αGalCer (αGC) or control vehicle. On day 2 postinfection, pancreatic islets were harvested and dissociated into a single-cell suspension, and cells were stained with surface antibodies directed against CD45, CD11c, 120G8, and CD11b. Cells were then sorted with a BD ARIA II sorter. A: Dot plots correspond to a representative staining in pancreatic islets with gates used for sorting. B: Total RNA was isolated from sorted populations from CVB4- and CVB4 + αGC–treated mice, and mRNA levels were measured by quantitative RT-PCR. Data are the mean ± SD of specific gene expression relative to GAPDH. Data were obtained from three independent experiments with three mice in each group. *P < 0.05 by Mann-Whitney U test. pDC, plasmacytoid DC.