FIG. 5.

iNKT-cell activation and cytokine production. iNKT cells were analyzed in Vα14 transgenic Proins2^−/− female mice inoculated with CVB4 or PBS and treated with αGalCer (αGC) or control vehicle. Pancreatic islets were harvested on day 2 of the treatment and dissociated into a single-cell suspension. Cells were stimulated with phorbol myristic acid/ionomycin in the presence of Brefeldin A for 4 h, and intracellular staining was performed. Dot plots correspond to a representative staining in pancreatic islets. Summary data (right) were obtained from three independent experiments with three mice in each group ± SD. *P < 0.05 by Mann-Whitney U test. Tet, tetramer.