FIG. 5.

Enhanced jejunal epithelial proliferation and GSK-3β/β-catenin signaling activation in HFD mice. A: Body weight of CD and HFD mice. B: Food intake in CD and HFD mice. C: H-E staining CD and HFD mice intestine. D: Quantification of the villi length. E: BrdU immunohistochemical staining of CD and HFD mice intestine. F: Quantification of BrdU-positive cells. G: β-Catenin, GSK-3β Ser⁹ phosphorylation, total GSK-3β, and cyclin D1 expression were detected by Western blot in CD and HFD mice jejunum; β-actin was used as a loading control. H, I, and J: Quantification of β-catenin, GSK-3β Ser⁹ phosphorylation, and cyclin D1 expression. Western blot data from at least three independent experiments and expressed as mean ± SEM; mice n = 6–8 per group. *P < 0.05, **P < 0.01. Scale bar, 50 µm.